Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10218388 | Transfusion and Apheresis Science | 2018 | 4 Pages |
Abstract
Cryopreservation for the long-term storage of platelets (PLTs) is a useful method to overcome the limits of platelet shortage. This is an in vitro prospective study to evaluate the count, viability, and function of buffy coat-derived pooled platelet concentrates (BC-PLTs), treated with dimethyl sulphoxide (DMSO) and cryopreserved (CRY BC-PLTs) at â80â°C with a modified Valeri method. PLTs were stored in 6% DMSO with a patented kit. Overall, 49 BC-PLTs from 245 healthy volunteer donors were prepared, cryopreserved, and analysed before and after 3, 6, and 9âmonths of storage. In flow cytometry, a statistically significant reduction in CD 42b (92.7â±â4.29% at T0 vs. 23.6â±â27.5% at T3, 16.38â±â12.54% at T6, and 17.3â±â9.6% at T9) and PAC-1 (1.9â±â1.34% at T0 vs. 0.62â±â0.4% at T3, 0.63â±â0.83% at T6, and 0.49â±â0.48% at T9) was observed after storage. CRY BC-PLTs showed a good and stable endogenous thrombin generation potential (nM min): 529.25â±â98.64 at T0 vs. 533.04â±â103.15 at T9 months. CRY BC-PLTs showed a good viability in vitro, according to currently accepted criteria for cryopreserved PLTs.
Keywords
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Authors
Mariasanta Napolitano, Salvatrice Mancuso, Lucio Lo Coco, Piera Stefania Arfò, Simona Raso, Giovanni De Francisci, Francesco Dieli, Nadia Caccamo, Amalia Reina, Alberto Dolce, Rosalia Agliastro, Sergio Siragusa,