Effect of flow pattern on cellulase deactivation in stirred tank bioreactors

In biochemical process industries, the dynamic environment within the bioreactor and in the purification equipment is known to affect the enzyme activity and yield of enzyme production. This has drawn our attention to examine the effect of various flow parameters on the deactivation behavior of enzyme in stirred tank reactor. In the present work, cellulase deactivation was investigated in 0.1,0.3 and 0.57 m i.d. stirred vessels with single and dual impeller. Enzyme solution was subjected to hydrodynamic stress using various types of impeller and impeller combinations over a wide range of power consumption (0.02