Article ID Journal Published Year Pages File Type
10275851 Journal of Electroanalytical Chemistry 2005 8 Pages PDF
Abstract
An effective promotion for the generation of superoxide anion radical from H2O2 and O2 was accomplished with montmorillonite K10 clay mineral (denoted Mont. K10). This catalytic event was characterized by a fluorescence assay using amplex red and superoxide dismutase (SOD) as probes. The iron species (FeII and FeIII) contained in Mont. K10 are thought to contribute to this catalytic event. Due to this property, a glucose sensor was fabricated on the basis of Mont. K10, amplex red, and glucose oxidase (GOx). When glucose is injected with amplex red over the clay|GOx electrode, H2O2 is produced in the enzyme zone, transduced to superoxide anion radical by the clay, and quenched by amplex red. Since the resulting product, resorufin, is emission-active under the irradiation of visible light (λex: 563 nm; λem: 583 nm, pH 8), the analyte can thus be recognized and reported as optical signals with a luminescence spectrometer via a Y-type optical fiber atop of the electrode. The optical signal showed a linear response to glucose up to 150 μM at pH 8. The lower and upper limits were estimated to be 1.0 and 150 μM, respectively, at this pH. These results suggest that Mont. K10 is a promising O2- transducer.
Related Topics
Physical Sciences and Engineering Chemical Engineering Chemical Engineering (General)
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