Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10276213 | Journal of Electroanalytical Chemistry | 2005 | 11 Pages |
Abstract
We report the characterization of a new bifunctional electroactive polymer, poly(5-hydroxy-1,4-naphthoquinone (juglone)-co-5-hydroxy-3-thioacetic acid-1,4-naphthoquinone), which can be used for direct electrochemical detection of DNA hybridization. The polymer structure was characterized based on molecular orbital calculations and ex situ and in situ FT-IR spectroscopy. The quinone group integrated in the polymer presents very stable electroactivity in neutral aqueous medium and can act as a redox marker for hybridization. The carboxylic function allows the preparation of oligonucleotides (ODN) probe-modified films by covalent binding. The mechanism of hybridization detection was studied by cyclic voltammetry and electrochemical impedance spectroscopy. The “signal-on” upon hybridization could be attributed to a change in conformation from random ODN coils on the probe-modified film to helix double stranded ODNs upon hybridization.
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Physical Sciences and Engineering
Chemical Engineering
Chemical Engineering (General)
Authors
B. Piro, J. Haccoun, M.C. Pham, L.D. Tran, A.Rubin A.Rubin, H. Perrot, C. Gabrielli,