| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 103500 | Legal Medicine | 2015 | 6 Pages |
•A total of 380 bacterial strains were isolated from soil samples using human blood agar.•Thirteen strains possessed α-galactosidase activity.•16S rRNA sequence analysis revealed a close relatedness to the genus Bacillus.•Indirect competitive ELISA confirmed seven strains showed type B antigen degradation.•Particular attention should be paid to the conversion of type B to O.
The current study investigated whether ABO blood mistyping of human biological samples is induced by soil bacteria. A total of 380 bacterial strains were isolated from 50 discrete soil samples using human blood agar, and glycosidase activity evaluated for all strains using 4-nitropheny glycosides (4-nitrophenyl n-acetyl-α-d-galactosaminide, 4-nitrophenyl-α-d-galactopyranoside, 4-nitrophenyl-α-l-fucopyranoside) as substrates. Thirteen strains possessed α-galactosidase activity, and 16S rRNA sequence analysis revealed a close relatedness to the genus Bacillus. An indirect competitive enzyme-linked immunosorbent assay confirmed seven strains exhibited type B antigen degradation activity. These results demonstrated that 1.8% of the bacteria isolated from soil, were Bacillus sp., possessed galactosidase activity, and had the potential to cause ABO blood mistyping.
