Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10372701 | Journal of Hazardous Materials | 2011 | 5 Pages |
Abstract
Phytolacca americana L. (pokeweed) is a promising species for Cd phytoextraction with large biomass and fast growth rate. To further understand the mechanisms involved in Cd tolerance and detoxification, the present study investigated subcellular distribution and chemical forms of Cd in pokeweed. Subcellular fractionation of Cd-containing tissues indicated that both in root and leaves, the majority of the element was located in soluble fraction and cell walls. Meanwhile, Cd taken up by pokeweed existed in different chemical forms. Results showed that the greatest amount of Cd was found in the extraction of 80% ethanol in roots, followed by 1Â M NaCl, d-H2O and 2% HAc, while in leaves and stems, most of the Cd was extracted by 1Â M NaCl, and the subdominant amount of Cd was extracted by 80% ethanol. It could be suggested that Cd compartmentation with organo-ligands in vacuole or integrated with pectates and proteins in cell wall might be responsible for the adaptation of pokeweed to Cd stress.
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Authors
Xiaoping Fu, Changming Dou, Yingxu Chen, Xincai Chen, Jiyan Shi, Mingge Yu, Jie Xu,