Article ID Journal Published Year Pages File Type
10374834 Colloids and Surfaces B: Biointerfaces 2011 9 Pages PDF
Abstract
▶ A biotinylated polymer, poly(lactide-co-2,2-dihydroxymethyl-propylene carbonate-graft-biotin) [P(LA-co-DHC/biotin)] was synthesized and served as a model polymer, on which co-existed non-specific protein adsorption and specific interaction. ▶ Three different treatment were used to modify the polymer film to let the films had different properties. ▶ A newly developed technology, Fourier transform surface plasmon resonance (FT-SPR) was utilized to study specific and non-specific interactions between proteins and a biotinylated polymer film by monitoring adsorptions of streptavidin (SAv) and bovine serum albumin (BSA) on the polymer films at the same time. ▶ The hydration treatment led to migration of the biotin moieties from inner film to surface and thus resulted in less BSA adsorption and more SAv binding. ▶ Blocking layer on the polymer surface saturated the active sites for physical and chemical adsorptions on the surface and thus the non-specific adsorption of BSA was the weakest, while the specific binding of SAv on the blocked film was relatively the highest.
Related Topics
Physical Sciences and Engineering Chemical Engineering Colloid and Surface Chemistry
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