Research design in digital luminance analysis

The prevalence of digital image analysis in studies spanning a wide range of fields makes the importance of set-up design decidedly important for repeatability and testing between studies, especially for those interested in the analysis of microscopic properties. This note outlines some important guidelines for setting up an image analysis protocol, and provides a case study based on the digital analysis of dental cement. Our method allowed for us (1) to get data through the conversion of optical luminance into numerical values and (2) to accurately measure image features through numerical analysis.