Looking for practical tools to achieve next-future applicability of dark fermentation to produce bio-hydrogen from organic materials in Continuously Stirred Tank Reactors

► Looking for application of H2-production from organic substrates in CSTR reactor. ► Acclimation of soil and digested slurry allowed obtaining efficient mixed inocula. ► Development of a methodology to obtain maximized bio-H2 potential in batch test. ► Organic waste gave the highest H2 potential production in batch test. ► Continuous fermentation of organic waste gave only 30% of its bio-H2 potential.