Repeated-batch fermentation of lignocellulosic hydrolysate to ethanol using a hybrid Saccharomyces cerevisiae strain metabolically engineered for tolerance to acetic and formic acids

► A yeast strain was metabolically engineered for tolerance to fermentation inhibitors. ► Moreover, the resistance was improved by a crossbreeding strategy. ► The recombinant diploid strain produced ethanol from lignocellulosic hydrolysate. ► Batch fermentation was repeated five times without any loss of fermentation ability.