Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10395024 | Bioresource Technology | 2011 | 5 Pages |
Abstract
The strategic design of this study aimed at producing succinate and polyhydroxyalkanoate (PHA) from substrate mixture of glycerol/glucose and fatty acid in Escherichia coli. To accomplish this, an E. coli KNSP1 strain derived from E. coli LR1110 was constructed by deletions of ptsG, sdhA and pta genes and overexpression of phaC1 from Pseudomonas aeruginosa. Cultivation of E. coli KNSP1 showed that this strain was able to produce 21.07Â g/L succinate and 0.54Â g/L PHA (5.62Â wt.% of cell dry weight) from glycerol and fatty acid mixture. The generated PHA composed of 58.7Â mol% 3-hydroxyoctanoate (3HO) and 41.3Â mol% 3-hydroxydecanoate (3HD). This strain would be useful for complete utilization of byproducts glycerol and fatty acid of biodiesel production process.
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Authors
Zhen Kang, Lili Du, Junhua Kang, Yang Wang, Qian Wang, Quanfeng Liang, Qingsheng Qi,