Multi-type hyper-spectral microscopic imaging system

We present a hyper-spectral microscopic imaging system capable of obtaining both transmission- and fluorescence-spectral images for biological measurements. For obtaining high quality images of samples with low self-fluorescence, large aperture optics and low vibration elements were implemented in the system. This study focused on obtaining different types of spectral information, i.e., transmission and fluorescence, to study the structure of the objects and observe the similarities and differences in composition at different positions. By employing this system to investigate bee pollen, we observed a special thin-film structure, which cannot be detected using conventional microscopic systems.