Integrated microdevice of reverse transcription-polymerase chain reaction with colorimetric immunochromatographic detection for rapid gene expression analysis of influenza A H1N1 virus

► We report a fully integrated microdevice incorporating a reverse transcription-polymerase chain reaction (RT-PCR) with an immunochromatographic strip (ICS) for visual detection of rapid gene expression of influenza A H1N1 virus on a portable system. ► An assembled five-layered glass-polydimethylsiloxane hybrid microdevice has a functional unit of a pneumatic micropump, a RT-PCR chamber, a microvalve, and an ICS. ► One-step RT-PCR reaction, gold nanoparticle conjugation, and the colorimetric detection on an ICS were performed successively on a portable system to identify the target H1 gene of H1N1 virus.