Detection of single-nucleotide polymorphisms with novel leaky surface acoustic wave biosensors, DNA ligation and enzymatic signal amplification

► We have developed a new technique based on a LSAW biosensor, enzymatic DNA ligation and enzymatic signal amplification for the detection of SNPs. ► Routine detection conditions are optimized. ► The detection range is from 10−6 mol/L to 10−12 mol/L (ΔP = 1.853 lg C + 22.235, r = 0.976). ► A single-point mutation at nucleotide A2293G in JEV was successfully detected by this technique.