Monitoring extracellular K+ flux with a valinomycin-coated silicon nanowire field-effect transistor

Article ID	Journal	Published Year	Pages	File Type
10429331	Biosensors and Bioelectronics	2012	7 Pages	PDF

Abstract

âº A silicon nanowire field-effect transistor coated with a polyvinyl chloride membrane containing valinomycin was used as a biosensor. âº Apparent association constants between valinomycin and Li+, Na+, K+, and Cs+ in Tris buffer solution were determined respectively. âº The nanowire-transistor biosensor was employed to detect the K+-efflux from live chromaffin cells. âº The nanowire-transistor biosensor is suitable for the applications in cellular recording investigations.

Keywords

VSD NMG ΔG Metal ion concentration Valinomycin K+ concentration TRIS ISD PLL N-methyl-d-glucamine nAChR PDMS SOI ACh Acetylcholine Buried oxide Field-effect transistor Tris(hydroxymethyl)aminomethane Association constant box Potassium efflux val Silicon-on-insulator SEM Scanning electron microscopy nanowire Electrical Conductance Poly-l-lysine Polydimethylsiloxane PVC Polyvinyl chloride nicotinic acetylcholine receptor Alkali metal ion