Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10429407 | Biosensors and Bioelectronics | 2011 | 5 Pages |
Abstract
In this paper, an electrochemical sensor for 17β-estradiol (E2) based on the molecular imprinting polymer (MIP) membranes had been constructed. 6-mercaptonicotinic acid (MNA) and E2 were first assembled on the surface of platinum nanoparticles-modified glassy carbon electrode (PtNPs/GCE) by the formation of Pt-S bonds and hydrogen-bonding interactions, and subsequently the polymer membranes were formed by electropolymerization. Finally, a novel molecularly imprinted sensor (MIS) was obtained after removal of E2. Experimental parameters such as deposition time, scan cycles, pH value and accumulation condition were optimized. Under optimal conditions, the MIS exhibited a large adsorption capacity and high selectivity. A good linearity was obtained in the range of 3.0 Ã 10â8-5.0 Ã 10â5 mol Lâ1 (r = 0.996) with an estimated detection limit of 1.6 Ã 10â8 mol Lâ1. MIS had been successfully used to analyze E2 in water samples without complex pretreatment. Meanwhile, the average recoveries were higher than 93.9% with RSD < 3.7%. All results above reveal that MIS is an effective electrochemical technique to determine E2 real-time in complicated matrix.
Related Topics
Physical Sciences and Engineering
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Authors
Lihua Yuan, Jun Zhang, Ping Zhou, Jiaxing Chen, Ruoyu Wang, Tingting Wen, Yun Li, Xuemin Zhou, Huijun Jiang,