Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10429408 | Biosensors and Bioelectronics | 2011 | 6 Pages |
Abstract
We describe analysis and control of 50S ribosomal subunits by a solid-state 45Â nm diameter nanopore incorporated in a microfluidic chip. When used as a resistive pulse sensor, translocation of single 50S subunits through the nanopore produces current blockades that have a linear dependence on applied voltage. Introduction of individual subunits into the fluidic channel shows a threshold behavior that allows controlled entry of individual 50S ribosomal subunits. The incorporation of nanopores into a larger optofluidic chip system opens possibilities for electrical and optical studies of single ribosomes in well-defined and rapidly variable chemical environments.
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Chemistry
Analytical Chemistry
Authors
Mikhail I. Rudenko, Matthew R. Holmes, Dmitri N. Ermolenko, Evan J. Lunt, Sarah Gerhardt, Harry F. Noller, David W. Deamer, Aaron Hawkins, Holger Schmidt,