Molecularly imprinted hydroxyapatite thin film for bilirubin recognition

A novel piezoelectric sensor has been developed for bilirubin (BR) detection, based on the modification of molecularly imprinted hydroxyapatite (HAP) film onto a quartz crystal by molecular imprinting and surface sol-gel technique. The performance of the developed BR biosensor was evaluated and the results indicated that a sensitive BR biosensor could be fabricated. The obtained BR biosensor presents high-selectivity monitoring of BR, better reproducibility, shorter response time (37 min), wider linear range (0.05-80 μM) and lower detection limit (0.01 μM). The analytical application of the BR biosensor confirms the feasibility of BR detection in serum sample.