Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10429489 | Biosensors and Bioelectronics | 2011 | 6 Pages |
Abstract
Simultaneous detection of multianalytes associated with a particular cancer is beneficial for disease diagnosis. Here, a facile immunosensing strategy was designed to allow simultaneous electrochemical detection of dual proteins, in a single run. CdSe and PbS water-soluble quantum dots (QDs) were prepared and coated on monodisperse silica nanoparticles as labels for proteins detection. Rabbit immunoglobulin G antigen (IgG) and carcinoembryonic antigen (CEA) were chosen as model proteins for analysis. After a typical sandwich immunoassay, CdSe and PbS QDs labels were introduced onto the Au substrates' surface, which were then dissolved and could be simultaneously monitored by square-wave-voltammetric (SWV) stripping measurements. Under selected conditions, IgG and CEA could be assayed in the ranges of 0.05-40 ng mLâ1 and 0.05-25 ng mLâ1, respectively. The proposed method possessed high sensitivity, good precision, and satisfactory reproducibility and regeneration.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Jing Qian, Haichao Dai, Xiaohu Pan, Songqin Liu,