Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10429521 | Biosensors and Bioelectronics | 2011 | 6 Pages |
Abstract
We present a novel portable immunochromatographic electrochemical biosensor (IEB) for simple, rapid, and sensitive biomonitoring of trichloropyridinol (TCP), a metabolite biomarker of exposure to organophosphorus insecticides. Our new approach takes the advantage of immunochromatographic test strip for a rapid competitive immunoreaction and a disposable screen-printed carbon electrode for a rapid and sensitive electrochemical analysis of captured HRP labeling. Several key experimental parameters (e.g. immunoreaction time, the amount of HRP labeled TCP, concentration of the substrate for electrochemical measurements, and the blocking agents for the nitrocellulose membrane) were optimized to achieve a high sensitivity, selectivity and stability. Under optimal conditions, the IEB has demonstrated a wide linear range (0.1-100Â ng/ml) with a detection limit as low as 0.1Â ng/ml TCP. Furthermore, the IEB has been successfully applied for biomonitoring of TCP in the rat plasma samples with in vivo exposure to organophosphorus insecticides like Chlorpyrifos-oxon (CPF-oxon). The IEB thus opens up new pathways for designing a simple, rapid, clinically accurate, and quantitative tool for TCP detection, as well as holds a great promise for in-field screening of metabolite biomarkers, e.g., TCP, for humans exposed to organophosphorus insecticides.
Related Topics
Physical Sciences and Engineering
Chemistry
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Authors
Limin Wang, Donglai Lu, Jun Wang, Dan Du, Zhexiang Zou, Hua Wang, Jordan N. Smith, Charles Timchalk, Fengquan Liu, Yuehe Lin,