Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10429530 | Biosensors and Bioelectronics | 2011 | 7 Pages |
Abstract
We have developed a stable and selective ATP biosensor for long-term in vitro tissue monitoring. The electrode was fabricated by entrapping glucose oxidase (GOx) and hexokinase (HEX) in a poly-phenol film on a Pt microelectrode. The biosensor was stable to a fixed concentration of glucose for over 20 min and had a limit of detection of 9.9 ± 3.2 nM, with a sensitivity of 45.8 ± 1.22 pA μMâ1. Most significantly of all, the response on the ATP biosensor did not alter in the presence of 1 mM ascorbic acid, 5 μM dopamine, 5 μM serotonin, 5 μM ADP and 5 μM AMP. The ATP biosensor was also shown to have excellent stability over 7 days, and showed only a 23.92 ± 3.55% loss in sensitivity. The ATP biosensor was utilised for the in vitro detection of ATP from gastrointestinal tissue. The ATP biosensor response was stable for 5 h during in vitro recordings from ileum tissue. ATP release was shown to be greater from the mucosal surface in the ileum compared to the colon.
Related Topics
Physical Sciences and Engineering
Chemistry
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Authors
Bhavik Anil Patel, Michelle Rogers, Talia Wieder, Danny O'Hare, Martyn G. Boutelle,