Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10429552 | Biosensors and Bioelectronics | 2011 | 8 Pages |
Abstract
The immobilisation unit and multiplex SPR analyser were respectively evaluated in their ability to be fit-for-purpose for binding partner attachment and detection of high and low molecular weight molecules. The multiplexing capability of the dual technology was assessed using phycotoxin concentration analysis as a model system. The parent compounds of four toxin groups were immobilised within a single chip format and calibration curves were achieved. The chip design and SPR technology allowed the compartmentalisation of the binding interactions for each toxin group offering the added benefit of being able to distinguish between toxin families and perform concentration analysis. This model is particularly contemporary with the current drive to replace biological methods for phycotoxin screening.
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Authors
Katrina Campbell, Terry McGrath, Stefan Sjölander, Thord Hanson, Mattias Tidare, Ãsten Jansson, Anna Moberg, Mark Mooney, Christopher Elliott, Jos Buijs,