Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10429594 | Biosensors and Bioelectronics | 2005 | 7 Pages |
Abstract
An ethanol biosensor based on electrogenerated chemiluminescence detection was developed. Electrogenerated chemiluminescence reagent tris(2,2â²-bipyridyl)ruthenium (II) and alcohol dehydrogenase were immobilized in the same sol-gel hybrid film. The copolymer poly(vinyl alcohol) with 4-vinylpyridine and cation exchanger Nafion were incorporated into sol-gel film to provide the microenvironment for retaining the activity of enzyme and immobilize tris(2,2â²-bipyridyl)ruthenium (II). The design was simpler than the previous two-layer format. The experimental conditions, such as scan rate, pH and concentration of the cofactor were investigated. The intensity of electrogenerated chemiluminescence increased linearly with ethanol concentration from 2.5Â ÃÂ 10â5 to 5.0Â ÃÂ 10â2Â M and detection limit was 1.0Â ÃÂ 10â5Â M. The prepared biosensor exhibited high sensitivity, wide linear range and good stability.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Zhiai Xu, Zhihui Guo, Shaojun Dong,