Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10429745 | Biosensors and Bioelectronics | 2005 | 6 Pages |
Abstract
A novel organic-inorganic nanocomposite of methylene blue (MB) and silicon oxide was synthesized and characterized by TEM, FTIR, and UV-vis. The as-prepared material was able to transfer the electron of the MB to electrode and was different from other SiO2 spheres structurally. It can be used as mediator to construct a biosensor with horseradish peroxidase (HRP) coimmobilized in the gelatine matrix and cross-linked with formaldehyde. The resulting biosensor exhibited fast amperometric response and good stability to hydrogen peroxide (H2O2). The linear range for H2O2 determination was from 1 Ã 10â5 to 1.2 Ã 10â3 M, with a detection limit of 4 Ã 10â6 M based on S/N = 3. Moreover, the lifetime is more than 3 months under dry conditions at 4 °C.
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Authors
Hui Yao, Nan Li, Shu Xu, Jin-Zhong Xu, Jun-Jie Zhu, Hong-Yuan Chen,