Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10429776 | Biosensors and Bioelectronics | 2005 | 7 Pages |
Abstract
Cell-based biosensors incorporate cells as sensing elements that convert changes in immediate environment to signals for processing. This paper reports an investigation on light-addressable potentiometric sensor (LAPS) to be used as a possible cell-base biosensor that will enable us to monitor extracellular action potential of single living cell under stimulant. In order to modify chip surface and immobilize cells, we coat a layer of poly-l-ornithine and laminin on surface of LAPS chip on which rat cortical cells are grown well. When 10 μg/ml acetylcholine solution is administrated, the light pointer is focused on a single neuronal cell and the extracellular action potential of the targeted cell is recorded with cell-based biosensor based on LAPS. The results demonstrate that this kind of biosensor has potential to monitor electrophysiology of living cell non-invasive for a long term, and to evaluate drugs primarily.
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Physical Sciences and Engineering
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Authors
Gaixia Xu, Xuesong Ye, Lifeng Qin, Ying Xu, Yan Li, Rong Li, Ping Wang,