Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10429946 | Biosensors and Bioelectronics | 2005 | 7 Pages |
Abstract
A sensitive and selective amperometric glucose biosensor based on platinum microparticles dispersed in nano-fibrous polyaniline (PANI) was investigated. Poly (m-phenylenediamine) (PMPD), which was employed as an anti-interferent barrier and a protective layer to platinum microparticles, was deposited onto platinum-modified PANI in the presence of glucose oxidase. The morphology of PANI, Pt/PANI and PMPD-GOD/Pt/PANI were investigated by scanning electron microscopy. The results show that PANI has a nano-fibrous morphology. The enzyme electrode exhibits excellent response performance to glucose with linear range from 2Ã10â6 to 12Ã10â3 M and fast response time within 7 s. Due to the selective permeability of PMPD, the enzyme electrode also shows good anti-interference to uric acid and ascorbic acid. The Michaelis-Menten constant km and the maximum current density imax of the enzyme electrode were 9.34Ã10â3 M and 917.43 μA cmâ2, respectively. Furthermore, this glucose biosensor also has good stability and reproducibility.
Related Topics
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Authors
Haihui Zhou, Hong Chen, Shenglian Luo, Jinhua Chen, Wanzhi Wei, Yafei Kuang,