Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10430099 | Biosensors and Bioelectronics | 2005 | 7 Pages |
Abstract
Screen-printed amperometric glucose biosensors have been fabricated using a water-based carbon ink. The enzyme glucose oxidase (GOD) and the electrocatalyst cobalt phthalocyanine were mixed with the carbon ink prior to the screen-printing process; therefore, biosensors are prepared in a one-step fabrication procedure. Optimisation of the biosensor performance was achieved by studying the effects of pH, buffer strength, and applied potential on the analytical response. Calibration studies were performed under optimum conditions, using amperometry in stirred solution, with an operating potential of +500 mV versus SCE. The sensitivity was found to be 1170 nA mMâ1, with a linear range of 0.025-2 mM; the former represents the detection limit. The disposable amperometric biosensor was evaluated by carrying out replicate determinations on a sample of bovine serum. This was achieved by the method of multiple standard additions and included a correction for background currents arising from oxidisable serum components. The mean serum concentration was calculated to be 8.63 mM and compared well with the supplier's value of 8.3 mM; the coefficient of variation was calculated to be 3.3% (n = 6).
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Eric Crouch, David C. Cowell, Stephen Hoskins, Robin W. Pittson, John P. Hart,