Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10430102 | Biosensors and Bioelectronics | 2005 | 7 Pages |
Abstract
A method for rapid sensitive detection of DNA or RNA was designed using a composite screen-printed carbon electrode modified with multi-walled carbon nanotubes (MWNTs). MWNTs showed catalytic characteristics for the direct electrochemical oxidation of guanine or adenine residues of signal strand DNA (ssDNA) and adenine residues of RNA, leading to indicator-free detection of ssDNA and RNA concentrations. With an accumulation time of 5 min, the proposed method could be used for detection of calf thymus ssDNA ranging from 17.0 to 345 μg mlâ1 with a detection limit of 2.0 μg mlâ1 at 3Ï and yeast tRNA ranging from 8.2 μg mlâ1 to 4.1 mg mlâ1. AC impedance was employed to characterize the surface of modified electrodes. The advantages of convenient fabrication, low-cost detection, short analysis time and combination with nanotechnology for increasing the sensitivity made the subject worthy of special emphasis in the research programs and sources of new commercial products.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Yongkang Ye, Huangxian Ju,