Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10430104 | Biosensors and Bioelectronics | 2005 | 9 Pages |
Abstract
An enzyme-based glucose biosensor modified to release nitric oxide (NO) via a xerogel microarray is reported. The biosensor design is as follows: (1) glucose oxidase (GOx) is immobilized in a methyltrimethoxysilane (MTMOS) xerogel layer; (2) a blended polyurethane/hydrophilic polyurethane coating prevents enzyme leaching and imparts selectivity for glucose; and (3) micropatterned xerogel lines (5 μm wide) separated by distances of 5 or 20 μm provide NO-release capability. This configuration allows for increased glucose sensitivity relative to sensors modified with NO-releasing xerogel films since significant portions of the sensor surface remain unmodified. Glucose diffusion to the GOx layer is thus less inhibited. The micropatterned NO-releasing biosensors generate sufficient NO levels to reduce both Pseudomonas aeruginosa and platelet adhesion without significantly compromising the enzymatic activity of GOx. The glucose response, linearity and stability of the NO-releasing micropatterned sensors are reported.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Bong Kyun Oh, Mary E. Robbins, Brian J. Nablo, Mark H. Schoenfisch,