Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10432151 | Journal of Biomechanics | 2012 | 7 Pages |
Abstract
During orthodontic tooth movement (OTM), alveolar bone is resorbed by osteoclasts in compression sites (CS) and is deposited by osteoblasts in tension sites (TS). The aim of this study was to develop a standardized OTM protocol in mice and to investigate the expression of bone resorption and deposition markers in CS and TS. An orthodontic appliance was placed in C57BL6/J mice. To define the ideal orthodontic force, the molars of the mice were subjected to forces of 0.1 N, 0.25 N, 0.35 N and 0.5 N. The expression of mediators that are involved in bone remodeling at CS and TS was analyzed using a Real-Time PCR. The data revealed that a force of 0.35 N promoted optimal OTM and osteoclast recruitment without root resorption. The levels of TNF-α, RANKL, MMP13 and OPG were all altered in CS and TS. Whereas TNF-α and Cathepsin K exhibited elevated levels in CS, RUNX2 and OCN levels were higher in TS. Our results suggest that 0.35 N is the ideal force for OTM in mice and has no side effects. Moreover, the expression of bone remodeling markers differed between the compression and the tension areas, potentially explaining the distinct cellular migration and differentiation patterns in each of these sites.
Related Topics
Physical Sciences and Engineering
Engineering
Biomedical Engineering
Authors
Silvana Rodrigues de Albuquerque Taddei, Adriana Pedrosa Moura, Ildeu Jr., Gustavo Pompermaier Garlet, Thiago Pompermaier Garlet, Mauro Martins Teixeira, TarcÃlia Aparecida da Silva,