Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10432232 | Journal of Biomechanics | 2012 | 8 Pages |
Abstract
Matrix metalloproteinase (MMP)-1, 2, with their endogenous inhibitors, tissue inhibitor of metalloproteinase (TIMP)-1, 2 are critical for extracellular matrix remodeling in human periodontal ligament (PDL) and their expression are sensitive to mechanical stresses. Shear stress as the main type of mechanical stress in tooth movement is involved in matrix turnover. However, how shear stress regulates MMPs and TIMPs system is still unclear. In this study, we investigated the effect of fluid shear stress on expression of MMP-1, 2 and TIMP-1, 2 in human PDL cells and the possible roles of mitogen-activated protein kinases in this process. Three levels of fluid shear stresses (6, 9 and 12Â dyn/cm2) were loaded on PDL cells for 2, 4, 8 and 12Â h. The results indicated that fluid shear stress rearranged cytoskeleton in PDL cells. Fluid shear stress increased expression of MMP-1, 2, TIMP-1 and suppressed TIMP-2 expression. MAP kinases including extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK) and p38 were activated rapidly by fluid shear stress. The ERK inhibitor blocked fluid shear stress induced MMP-1 expression and P38 inhibitor reduced fluid shear stress stimulated MMP-2 expression. Our study suggested that fluid shear stress involved in PDL remodeling via regulating MMP-1, 2 and TIMP-1, 2 expression. ERK regulated fluid shear stress induced MMP-1 expression and P38 play a role in fluid shear stress induced MMP-2 upregulation.
Keywords
ECMEGR-1AP-1PGE2PDLPDL cellsMIP-1αIL-8TGF-βMMPNF-κBBSAMAPKADAMAlbumin from bovine serumMechanical stressSDS-PAGESodium dodecyl sulfate polyacrylamide gel electrophoresisInterleukin-8Transforming Growth Factor BetaPeriodontal ligamentTIMPnuclear factor kappa-light-chain-enhancer of activated B cellsExtracellular matrixmatrix metalloproteinaseTissue inhibitors of metalloproteinaseMacrophage inflammatory protein-1αactivator protein 1early growth response protein 1Prostaglandin E2
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Authors
Lisha Zheng, Yan Huang, Wei Song, Xianghui Gong, Meili Liu, Xiaolin Jia, Gang Zhou, Luoping Chen, Ang Li, Yubo Fan,