Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10435396 | Medical Engineering & Physics | 2005 | 6 Pages |
Abstract
Due to the limited number of cells available in endothelial cell (EC) seeding of small diameter vascular grafts, high seeding rate and ideal proliferation are normally required and can be achieved by optimizing the EC seeding and culture procedures. In this study, by using rotational seeding at 0.16Â rpm for 12Â h in an incubator, 90% cells were successfully seeded on the polyurethane vascular grafts. Following a period of 72Â h of static culture, the cell retention after 6Â h of flushing could reach 90%. The retention was further enhanced after perfuse culture (9Â cm/s). The optimal procedures to prepare a polyurethane vascular graft (4-mm i.d., 4Â cm long) populated with firmly attached EC were therefore: (1) seeding the graft with 0.5Â ml of cell suspension containing â¼105 cells rotated at 0.16Â rpm for 12Â h; (2) culturing the seeded graft in static for 72Â h; and (3) culturing the graft by perfusion (9Â cm/s) for another 72Â h to 7 days. These procedures consistently resulted in a graft covered with confluent vein EC that fully retained on the surface after 6Â h of in vitro flushing.
Related Topics
Physical Sciences and Engineering
Engineering
Biomedical Engineering
Authors
Shan-hui Hsu, I-jine Tsai, Da-jun Lin, David C. Chen,