LPS-induced CCL2 expression and macrophage influx into the murine central nervous system is polyamine-dependent

Article ID	Journal	Published Year	Pages	File Type
10454974	Brain, Behavior, and Immunity	2011	11 Pages	PDF

Abstract

âº Ornithine decarboxylase (ODC) is the rate-limiting step in polyamine synthesis. âº LPS challenge promotes ODC expression by CNS neurons, glia & perivascular cells. âº Inhibiting ODC decreases CNS CCL2 expression and influx of pro-inflammatory macrophages. âº Injection of polyamines into the brain is sufficient to trigger macrophage infllux.âº Thus CNS cells regulate immune cell responses to pathogenic insults via polyamines.

Keywords

AMPA TNF Immune cell trafficking ODC DFMO Ornithine decarboxylase Hprt N-methyl-d-aspartic acid CNP TLR α-amino-3-hydroxyl-5-methyl-4-isoxazole-propionate NMDA iNOS LPS qPCR DMEM SPF FBS sSAT ApoD Apolipoprotein D Neuroinflammation interleukin Neurodegeneration Toll-like receptor autoimmunity CNS fetal bovine serum inducible nitric oxide synthase central nervous system tumor necrosis factor phycoerythrin TREM lipopolysaccharide Macrophage Microglia hypoxanthine phosphoribosyl transferase quantitative real-time PCR specific pathogen free Chemokine triggering receptor expressed on myeloid cells