Article ID Journal Published Year Pages File Type
10532577 Analytical Biochemistry 2013 5 Pages PDF
Abstract
The detection of rare mutations is particularly essential in many areas of biomedical research. Here, we report an ultrasensitive method to detect extremely rare point mutations based on electrochemiluminescent assay. The point mutation among large excess wild-type alleles is exclusively amplified through ligase detection reaction. The products corresponding to the amplification of mutant alleles are selectively captured by magnetic beads and then labeled with electrochemiluminescent substrates. Thus, point mutations with a percentage as small as 0.01% in the DNA population can be detected by electrochemiluminescent assay. Moreover, because the electrochemiluminescent signal of the mutation is proportional to the percentage of mutant alleles in the DNA population, the percentage of mutant alleles can be roughly accessed.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
Authors
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