Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10532589 | Analytical Biochemistry | 2013 | 5 Pages |
Abstract
Due to high sensitivity and good selectivity, electrochemical immunosensor is often used to detect tumor markers. Prostate-specific antigen (PSA) is the most validated tumor marker for prostate cancer. In this work, mesoporous silica nanoparticles (MSNs) were used to increase the fixation capacity of primary antibody, and silver nanoparticles (Ag NPs) were used to enhance the electron transfer rates. Silver hybridized mesoporous silica nanoparticles (Ag@MSNs) were synthesized and used as electrode material. Hydroquinone (HQ) generated a stable electrochemical signal and was used as a mediator. Based on the specific antibody-antigen interaction, a label-free immunosensor was developed for the sensing of PSA. The current method allows us to detect PSA over a wide concentration range from 0.05 to 50.0 ng mlâ1 with a detection limit of 15 pg mlâ1. The proposed immunosensor was used to determine PSA in human serum with satisfactory results.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Huan Wang, Yong Zhang, Haiqin Yu, Dan Wu, Hongmin Ma, He Li, Bin Du, Qin Wei,