Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10532596 | Analytical Biochemistry | 2013 | 6 Pages |
Abstract
We have devised protein-folding sensors that link protein stability to TEM-1 β-lactamase activity. The addition of osmolytes and other compounds with chemical chaperone activity to the growth medium of bacteria containing these sensors increases β-lactamase activity up to 207-fold in a dose-dependent manner. This enables the rapid detection and sensitive quantification of compounds that enhance in vivo protein stability.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Tsinatkeab T. Hailu, Linda Foit, James C.A. Bardwell,