Article ID Journal Published Year Pages File Type
10532623 Analytical Biochemistry 2011 6 Pages PDF
Abstract
Here we developed a simple set-and-mix assay to perform high-throughput screening of protein kinase A (PKA) inhibitors from the LOPAC 1280 compound library. This assay is based on the color change of gold nanoparticles on aggregation induced by a cationic substrate peptide as coagulant. In spite of the simplicity of this assay system, this assay can be applied to drug screening based on cellular kinases. We successfully found several highly active inhibitors, including compounds that have not been reported before.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
Authors
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