Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10532629 | Analytical Biochemistry | 2011 | 4 Pages |
Abstract
The development of a general 1-Zn(II) nucleoside diphosphate (NDP) sensor assay for rapid evaluation of glycosyltransferase (GT) activity is described. The 1-Zn(II) NDP sensor assay offers submicromolar sensitivity, compatibility with both purified enzymes and crude cell extracts, and exquisite selectivity for NDPs over the corresponding NDP-sugars. Thus, the 1-Zn(II) NDP sensor assay is anticipated to offer broad applicability in the context of GT engineering and characterization.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Hyun Soo Lee, Jon S. Thorson,