| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10532629 | Analytical Biochemistry | 2011 | 4 Pages |
Abstract
The development of a general 1-Zn(II) nucleoside diphosphate (NDP) sensor assay for rapid evaluation of glycosyltransferase (GT) activity is described. The 1-Zn(II) NDP sensor assay offers submicromolar sensitivity, compatibility with both purified enzymes and crude cell extracts, and exquisite selectivity for NDPs over the corresponding NDP-sugars. Thus, the 1-Zn(II) NDP sensor assay is anticipated to offer broad applicability in the context of GT engineering and characterization.
Related Topics
Physical Sciences and Engineering
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Authors
Hyun Soo Lee, Jon S. Thorson,