| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10532766 | Analytical Biochemistry | 2010 | 6 Pages |
Abstract
Separation of milligram amounts of heparin oligosaccharides ranging in degree of polymerization from 4 to 32 is achieved within 6Â h using continuous elution polyacrylamide gel electrophoresis (CE-PAGE) on commercially available equipment. The purity and structural integrity of CE-PAGE-separated oligosaccharides are confirmed by strong anion exchange high-pressure liquid chromatography, electrospray ionization Fourier transform mass spectrometry, and two-dimensional nuclear magnetic resonance spectroscopy. The described method is straightforward and time-efficient, affording size-homogeneous oligosaccharides that can be used in sequencing, protein binding, and other structure-function relationship studies.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Tatiana N. Laremore, Mellisa Ly, Kemal Solakyildirim, Dmitri V. Zagorevski, Robert J. Linhardt,