Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10532819 | Analytical Biochemistry | 2013 | 24 Pages |
Abstract
A sensitive amperometric acetylcholinesterase (AChE) biosensor based on platinum nanoparticles (Pt NPs), carboxylic graphene (CGR), and nafion (NF)-modified glassy carbon electrode (GCE) has been developed. The Pt NPs-CGR-NF nanocomposites with excellent conductivity, catalysis, and biocompatibility offered an extremely hydrophilic surface for AChE adhesion. Chitosan (CS) was used as cross-linker to immobilize the AChE on Pt-CGR-NF-modified GCE. NF was used as a protective membrane of the AChE biosensors. The AChE biosensor showed favorable affinity to acetylthiocholine chloride (ATCl) and could catalyze the hydrolysis of ATCl with an apparent Michaelis-Menten constant value of 148 μM. Under optimum conditions, the biosensor detected methyl parathion in the linear range from 1.0 Ã 10â13 to 1 Ã 10â10 M and from 1.0 Ã 10â10 to 1 Ã 10â8 M with a detection limit of 5 Ã 10â14 M and detected carbofuran in the linear range from 1.0 Ã 10â12 to 1 Ã 10â10 M and from 1.0 Ã 10â10 to 1 Ã 10â8 M with a detection limit of 5 Ã 10â13 M. The biosensor exhibited good sensitivity, acceptable stability, and reproducibility, thus providing a promising tool for analysis of enzyme inhibitors.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Long Yang, Guangcan Wang, Yongjun Liu,