Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10532866 | Analytical Biochemistry | 2010 | 9 Pages |
Abstract
We evaluated the performance of Fujifilm's new AP-3000 surface plasmon resonance biosensor for kinetic analysis and fragment screening. Using carbonic anhydrase II as a model system, we characterized a set of 10 sulfonamide-based inhibitors that range in molecular mass from 98 to 341Â Da and approximately 10,000-fold in affinity (0.4Â mM to 20Â nM). Although the data collected from the AP-3000 were generally similar to those collected using a Biacore T100, the AP-3000's stop-flow analyte delivery system complicated the shapes of the association- and dissociation-phase binding responses. We illustrate how reasonable estimates of the kinetic rate constants can be extracted from AP-3000 data by limiting data analysis to only the regions of the responses collected during flow conditions. We also provide an example of the results obtained for a fragment-screening study with the AP-3000, which is the ideal application of this technology.
Related Topics
Physical Sciences and Engineering
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Analytical Chemistry
Authors
Rebecca L. Rich, David G. Myszka,