Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10532932 | Analytical Biochemistry | 2010 | 6 Pages |
Abstract
Rapid amplification of cDNA ends (RACE) is an established strategy used to determine the transcription start point(s) and the 5â² untranslated region(s) of mRNA. Different approaches to perform 5â² RACE are available, and one particularly simple and powerful strategy is based on a phenomenon called template-switching. We investigated different aspects of template-switch-based 5â² RACE, and we describe the different steps leading to the in-house development of a complete 5â² RACE system-from oligonucleotide design to polymerase chain reaction (PCR) amplification. We show that the resulting system is reliable, time-efficient, and inexpensive.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Fernando Lopes Pinto, Peter Lindblad,