| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10533009 | Analytical Biochemistry | 2005 | 8 Pages |
Abstract
A homogeneous, fluorescence resonance energy transfer (FRET)-based DNA polymerase assay that is suitable for high-throughput screening for inhibitors, and can also be used for steady-state kinetic investigations, is described. The activity, kinetic mechanism, and processivity of the isolated α subunit of DNA polymerase III, the product of the dnaE gene, from the gram-negative pathogen Haemophilus influenzae were investigated using the FRET assay.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Adam Shapiro, Olga Rivin, Ning Gao, Laurel Hajec,