| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10533057 | Analytical Biochemistry | 2005 | 4 Pages |
Abstract
Undecaprenyl diphosphate-MurNAc-pentapeptide-GlcNAc (lipid II) is extracted from Escherichia coli cells by utilizing its unusual pH-dependent solubility property in a Bligh-Dyer system, and identified by electrospray ionization mass spectrometry in conjunction with a novel 15N mass shift analysis. The described approach will facilitate the structural characterization of lipid II variants from diverse bacteria, including antibiotic-resistant mutants, as well as the numerous minor uncharacterized lipids present in all biological systems.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ziqiang Guan, Steven D. Breazeale, Christian R.H. Raetz,