Article ID Journal Published Year Pages File Type
10533208 Analytical Biochemistry 2005 5 Pages PDF
Abstract
Proinsulin C-peptide was electroimmobilized to a quartz crystal microbalance sensor chip, localizing this low-pI peptide for covalent attachment to activated surface carboxyl groups. The resulting chip was used in a continuous flow biosensor to capture anti-C-peptide antibodies, which could subsequently be eluted in 5% formic acid between air bubbles for efficient recovery and mass spectrometric identification. The method is reproducible through repeated cycles, providing affinity purification of proteins under real-time monitoring of the binding and elution processes.
Related Topics
Physical Sciences and Engineering Chemistry Analytical Chemistry
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