Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10533208 | Analytical Biochemistry | 2005 | 5 Pages |
Abstract
Proinsulin C-peptide was electroimmobilized to a quartz crystal microbalance sensor chip, localizing this low-pI peptide for covalent attachment to activated surface carboxyl groups. The resulting chip was used in a continuous flow biosensor to capture anti-C-peptide antibodies, which could subsequently be eluted in 5% formic acid between air bubbles for efficient recovery and mass spectrometric identification. The method is reproducible through repeated cycles, providing affinity purification of proteins under real-time monitoring of the binding and elution processes.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Ermias Melles, Henrik Anderson, Daniel Wallinder, Jawed Shafqat, Tomas Bergman, Teodor Aastrup, Hans Jörnvall,