Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10533276 | Analytical Biochemistry | 2005 | 9 Pages |
Abstract
A cryogenic optical waveguide spectrometer that uses a Teflon-AF 2400 liquid core waveguide is described. In comparison to standard low-temperature absorption techniques, the liquid core waveguide approach not only affords the use of microliter samples but also provides significant improvements in sensitivity. Here we show low-temperature absorption spectra of various flavoproteins, including DNA photolyase, measured using this new technique. The technique has high reproducibility and can afford the detection of 15Â ng of flavoprotein. In addition, the technique requires several hundredfold less protein than standard low-temperature techniques for the same sensitivity. The performance of the spectrometer in the ultraviolet (UV) region is investigated experimentally and compared with standard UV absorption techniques. Results indicate that, below 300Â nm, the observed absorbances deviate from the Beer-Lambert law.
Keywords
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
M. Salim U. Siddiqui, Robert J. Stanley,