| Article ID | Journal | Published Year | Pages | File Type |
|---|---|---|---|---|
| 10533316 | Analytical Biochemistry | 2005 | 6 Pages |
Abstract
A simple, highly selective, and sensitive method has been developed to quantify methylation of DNA extracted from human peripheral blood mononuclear cells. Assay has been performed at nucleobases level. Cytosine and 5-methylcytosine DNA content has been detected by gas chromatography-mass spectrometry using [2-13C]cytosine and [2-13C]5-methylcytosine as internal standards. The methylation level has been calculated as 5-methylcytosine/total cytosine ratio. The working range selected on calibration curve, obtained by evaluation of standards and matrix-added standards measurements, is suitable for 5 μg DNA analysis. In this range, healthy human DNA methylation percentage is within 5-6%.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Antonella San Romerio, Giovanni Fiorillo, Ileana Terruzzi, Pamela Senesi, Giulio Testolin, Alberto Battezzati,