Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10533335 | Analytical Biochemistry | 2005 | 4 Pages |
Abstract
Avidin functional affinity electrophoresis (AFAEP) is a new method of affinity electrophoresis. In this technique, bifunctional linker glutaraldehyde is added to the polyacrylamide gel solution to embed avidin within the gel matrix by interaction with the amino/amide groups. Samples are heated with triglycine sodium dodecyl sulfate (SDS) sample buffer to ensure that biotinylated proteins biotinylated peptides are negatively charged and migrate electrophoretically toward the cathode through the avidin zone regardless of their pI values. The AFAEP method allows the capture and concentration of biotinylated proteins or biotinylated peptides irrespective of the use of SDS in both the sample buffer and the gel running buffer.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Bao-Shiang Lee, Shalini Gupta, Sangeeth Krishnanchettiar, Syed Salman Lateef,