Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10533938 | Analytical Biochemistry | 2005 | 8 Pages |
Abstract
This paper presents a simple assay for the direct quantification of small-sized (up to 1000 bp) fragmented DNA based on the differential separation between large- and small-sized DNA by polyethylene glycol precipitation. The assay can be applied to as low as 1 μg mlâ1 DNA and has a very low DNA detection limit (0.01 μg mlâ1 fragmented DNA), which can be further lowered at least 10-fold with anion exchange chromatography. The assay quantifies for the first time a more direct indicator of the extent of DNA damage than the usually assessed DNA nicks and base modifications, since small-sized fragmented DNA represents an unrepairable DNA damage of necrotic and apoptotic events that are related to normal and abnormal biological conditions.
Related Topics
Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Christos D. Georgiou, Nikolaos Patsoukis, Ioannis Papapostolou,