Article ID | Journal | Published Year | Pages | File Type |
---|---|---|---|---|
10536226 | Analytical Biochemistry | 2005 | 9 Pages |
Abstract
Laser ablation-inductively coupled plasma-mass spectrometry (LA-ICP-MS) has been developed as a new strategy for detection and imaging of β-amyloid protein in immunohistochemical sections from the brains of a transgenic mouse model of Alzheimer's disease. The distribution of β-amyloid deposits in tissue was based on measurement of Eu- and Ni-coupled antibodies. The laser-based methodologies (spot ablation, single line raster, and two-dimensional imaging) were also used to detect and map trace element distributions and thus provide a novel probe for both elemental and protein data. We also report the combination of laser capture microdissection with LA-ICP-MS as an alternative strategy for microanalysis of immunohistochemical sections.
Keywords
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Physical Sciences and Engineering
Chemistry
Analytical Chemistry
Authors
Robert W. Hutchinson, Alan G. Cox, Cameron W. McLeod, Peter S. Marshall, Alex Harper, Emma L. Dawson, David R. Howlett,